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For each pulldown or binding assay, 1 mg of lysate or 1 g of recombinant YBX1HA protein was diluted in the reaction buffer containing protease inhibitor (APExBIO, 1007), RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and 260 l of 2 TENT buffer (20 mM Tris-HCl pH 8.0, 2 mM EDTA, 500 mM NaCl and 1% v/v Triton X-100) and polysome extraction buffer (20 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 and 0.5% NP-40) in a final volume of 520 l

Learn how injectable BPC-157 may accelerate lumbar spine injury recovery

It is not intended for human consumption, injection, or use as a drug, food, or cosmetic

Best Practices After Reconstitution: Refrigerate immediately after reconstitution Avoid unnecessary agitation Protect solutions from excessive light exposure Minimize repeated temperature fluctuations Maintain detailed laboratory records Follow established handling procedures Important: If cloudiness, discoloration, or visible particulate matter appear, researchers should review laboratory protocols and handling procedures before continuing use

Molecular Details Peptide Sequence: Aib-His-D-2Nal-D-Phe-Lys Molecular Formula: CHNO Molecular Weight: 711.868 g/mol PubChem CID: 9831659 CAS Number: 170851-70-4 Source: PubMed Key Research Areas and Potential Benefits 1